Cardiac tissues were washed with cold phosphate buffered saline (PBS), and fixed with EM Grade 4% glutaraldehyde in 0.1 M
cacodylate buffer (pH 7.4).
The fixation of each larva was performed using 2.5% glutaraldehyde solution and 0.1 M
cacodylate buffer in pH 7.2, followed by storage at 5[degrees]C up to dehydration.
Gill fragments were fixed with 2.5 % glutaraldehyde in sodium
cacodylate buffer (0.1 M, pH 7.2) for 2 hours, refrigerated.
In brief, we fixed cells for 1 h with 2.5% glutaraldehyde in a 0.1 mmol/L sodium
cacodylate buffer and washed them with a mixture of 0.2 mmol/L saccharose and 0.1 mmol/L sodium
cacodylate.
schneideri toad (50 [micro]g/mL) or at basal condition when preparations were incubated with Tyrode solution (control) or methanolic extract for 5 min or 60 min (n = 3/group), were washed with the vehicle and then immersed in Karnovsky fixative (2% paraformaldehyde, 2.5% glutaraldehyde in 0.1 M sodium
cacodylate buffer, pH 7.2).
Urinary EVs were fixed in 2.5% glutaraldehyde in 0.1 M
cacodylate buffer for 2 hours at 4[degrees]C followed by 2 washes with 0.1 M
cacodylate buffer, 5 minutes each.
At the end of the cell culture studies, scaffolds were fixed with 2.5% (v/v) glutaraldehyde solution in 0.1 M sodium
cacodylate buffer for 1h at 4[degrees]C, washed with sodium
cacodylate buffer, and then dehydrated at room temperature in a gradient ethanol series up to 100%.
Briefly, the paraffin was removed with xylene from a 25 [micro]-thick section, followed by running the sample through a graded series of ethanols, and fixation in 2.5% glutaraldehyde/1% paraformaldehyde in 0.1 M
cacodylate buffer for 30 minutes at 4[degrees]C.
Six days later, the rats were anaesthetized and perfused with 4% formaldehyde in 0.1 M sodium
cacodylate buffer, pH 7.3.
The pellet was fixed in 1 ml of
cacodylate buffer (0.2 M sodium
cacodylate, 0.2 M HCl, pH 7.4) supplemented with 2.5% glutaraldehyde and incubated 8-10 h at room temperature (RT).