Thidiazuron: A potent
cytokinin for efficient plant regeneration in Himalayan popular (Populus ciliata Wall) using leaf explants.
In contrast to the results obtained from epicotyl explants, the presence of
cytokinin in the medium significantly affected the number of shoots formed from hypocotyl segments treated with BA at culture initiation and first subculture (F Test, P [less than or equal to] 0.05, Figures 3A and 3B).
The media investigated were the MS medium supplemented with single
cytokinin at various concentrations, 5, 10, 15 and 20 [micro]M, MS medium supplemented with single
cytokinin (5, 10, 15 and 20 [micro]M) in combination with 0.5 [micro]M 3-indolebutyric acid (IBA) and MS medium supplemented with single
cytokinin (5, 10, 15 and 20 [micro]M) in combination with 0.5 [micro]M IBA and 50 [micro]M adenine sulphate (ADS).
Increased concentration of this
cytokinin exhibited reduced rate of bud breaks and shoot growth for both cultivars.
Effects of
Cytokinin and Auxin on Shoot Organogenesis from Two Different Types of Explants.
There was no sign of shoot proliferation when shoot bud explants were cultured in media without
cytokinin and auxin.
In some plants the exogenous application of
cytokinin overcomes the inhibitory action of ABA in seed germination [11 and 12].
Thus, this study aims to determine the following parameters: direct shoot regeneration from nodal explants of Phalaenopsis violacea flower stalks on half-strength Murashige and Skoog (MS, 1962) medium with different growth regulators; the effect of different
cytokinin concentrations on PLB induction from leaf segments of Phalaenopsis violacea; and the effect of different
cytokinin concentrations on PLB proliferation rate (%) of Phalaenopsis violacea.
In tissue culture, the plant hormones auxin and
cytokinin are used in an agarbased medium, along with vitamins and plant nutrients.
The physiological effects of the resulting abnormally high
cytokinin levels include stunted growth, adventitious shoot formation (loss of apical dominance), delayed senescence, and reduced root formation.
What did the trick was a mild auxin mixed with a strong
cytokinin, one used commercially for defoliating cotton plants to ease harvesting.