Moderate hyperplasia of the bronchial epithelium was present with increased numbers of goblet cells and a small number of embryonated nematode eggs measuring 40 x 30 [micro]m within parabronchi and atria (Figs 2 and 3).
The methanolic extracts were made and concentrated by rotary evaporator and finally dissolved in distilled water before taking their antiviral trials in 7-11 days old chicken embryonated eggs.
Humans normally become infected by ingestion of embryonated eggs (each containing a fully developed larva, L2) from contaminated sources (soil, fresh or unwashed vegetables, or improperly cooked paratenic hosts).
Pilot studies are used to identify the relative suitability of differing cell-lines and embryonated eggs from differing specific-pathogen free (SPF) chicken flocks by investigating dosing titers and incubation temperatures and times.
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