Three ml blood was poured into the lithium
heparinized tubes and stored at 4AdegC for estimation of micronucleus frequency by CBMN assay.
Heparinized whole blood (2.0 mL) was added to tubes containing saline or tirofiban (263 ng/mL final concentration) and gently mixed.
Whole
heparinized blood was analyzed using a VetScan VS2 with Avian/Reptilian Profile Plus reagent rotors.
Randomly selected 7 of the fish from each replicate tanks were removed after 24 h, and they were bled from the caudal vein using
heparinized needles.
For assessment of biological parameters, 5 mL of fasting venous blood samples was taken under all aseptic conditions by venipuncture in the antecubital vein in
heparinized tubes.
The point-of-care analyzer utilizes a two site enzyme linked immunosorbent assay which tests
heparinized, whole blood.
Blood samples were collected via venipuncture from the jugular vein at 09:00 h and 15:00 h on d 7 from the start of the thermal exposure, and the blood samples were collected into a non-anti-coagulant, a
heparinized and an ethylenediaminetetraacetic acid (EDTA) coated vacutainers (BD, North Ryde, NSW, Australia).
The catheter was connected to a handmade water column system filled with
heparinized saline (Figure 1A), or to a multiparameter monitor (LW8, Life Window Lite, Digicare[R], Boynton Beach, FL, USA) via a pressure transducer filled with
heparinized saline (Figure 1B).
Louis, Missouri) was prepared as needed by dissolving in
heparinized Cortland's solution.
On the first day following admission, blood tests showed persistent hyperleukocytosis associated with severe hyperkalemia [cobas 8000 (Roche Diagnostics), indirect potentiometry on
heparinized plasma], which was not consistent with clinical features.
Heparinized antecubital blood samples (3-4 mL) were obtained from patients and control subjects for whole-blood cultures of human lymphocytes and determination of plasma 8-OHdG levels.