In gel-filtration column chromatography, immunoassayable hK13 of tissue extracts consistently eluted in a peak corresponding to a molecular mass of ~30 kDa, consistent with the molecular mass of free (noncomplexed) hK13.
The tumor marker assays were also performed on the contents of 5 separator-gel tubes filled with doubly distilled water and 5 tubes filled with serum containing no immunoassayable mucins; these measurements were performed after 72 h of contact between liquids and gels.
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