F, forward; IS,
insertion sequence; Prn, pertactin; R, reverse.
In this case, a nested PCR was used to target the multi-copy IS6110
insertion sequence, which is present in most Mycobacterium tuberculosis complex (MTBC) pathogens, these being the main causative agents of tuberculosis in humans and other animals (e.g., Muller et al., 2014).
The purpose of this study was to evaluate the efficacy of PCR tests targeting two different DNA sequences –
insertion sequence 6110 (IS6110) and protein antigen b (Pab), in the same set of clinical samples from osteoarticular TB cases, and to evaluate if the sensitivity of the assay is improved.
[7-12] The genetic location of [bla.sub.OXA-181] is typically on a Tn2013 transposon, immediately downstream of the
insertion sequence ISEcplB, carried on a 7.6-Kb, ColE-type plasmid.
Sequencing analysis of the ompk35 and ompk36 genes revealed the presence of the
insertion sequence IS1 between the promoter region and the start codon of the ompK35 gene, and the presence of the IS908 disrupting the ompK36 gene.
adiculatus lahorensis contains
insertion sequence (AACTGGGCTTCTCCTTAT) in the second half of V4 region (Fig.
Guzman, 2013, SNPs and an
insertion sequence in five Wx-A1 alleles as factors for variant Wx-A1 protein in wheat.
pseudomallei due to a process called reductive evolution wherein a massive invasion of many
insertion sequence (IS) elements altered the B.
We have investigated the presence and copy number of this
insertion sequence in M.
Each DD genotype was verified by a second PCR with primers specific to the
insertion sequence (K.
The assay was based on the amplification of a 181-bp fragment of the repetitive
insertion sequence I5481, which has been described in B.