Inhibition rates of germination rate of 3 d and seedling root length of 6 d of Italian ryegrass treated by
petroleum ether extract were 65.14% and 93.75%, respectively; significantly higher than the treatments of ethyl-acetate extract, n-butanol extract and residue (Pless than 0.05).
Extraction and Fractionation of Antioxidants: About 250 gm shade-dried ground whole plant was extracted successively with non- polar and polar solvents like
petroleum ether chloroform and methanol and water by maceration for 8 days in each of the solvents respectively.
Saponification of
Petroleum Ether Extracts: (Tsuda et al., '960):
Flavonoids were detected in all the leaf extracts & in stem it is present in
petroleum ether and ethanol extracts only.
The ability of different solvents to extract TPC was found asfollows: absolute methanol > 90% methanol > chloroform > acetone > 95% methanol > ethylacetate > nbutanol >
petroleum ether. The effect of different solvents on TFC values was found in the following order: absolute methanol > 95% methanol > chloroform > acetone > 90% methanol > ethylacetate > n-butanol >
petroleum ether.
The antioxidant activities of successive extracts of broccoli (
petroleum ether, chloroform, ethyl acetate and ethanol) and the crude extract were estimated by inhibiting the stable free radical.
Least activity was exhibited by
petroleum ether extract.
The [H.sub.2]O suspension was extracted in turn with
petroleum ether (117g) and [CH.sub.2][C1.sub.2] (600g), EtOAc (18g) and n-BuOH (26g).
Sixteen grams (0.12 mol) anhydrous aluminum chloride, 28 ml (0.1 mol) dry dodecyl-benzene and 30 ml
petroleum ether were put into a 250 ml fournecked flask fitted with a calcium chloride drying tube.
Among the various extracts, aqueous extracts showed the presence of significant amount of phytochemicals followed by methanol, ethanol, water, chloroform, and
petroleum ether extract and this may contribute to the better antibacterial activity.
Generally, using chloroform and methanol to extract cerebroside from the tissues will cause losses in biological activity, so we used supercritical carbon dioxide to extract the liposoluble constituent of cervus nippon antler velvet layer and extracted cerebroside with
petroleum ether. Meanwhile, silica gel column was used to purify cerebroside and thin layer chromatography (TLC) was used to identified cerebroside.