Nissl stains the rough endoplasmic reticulum and polyribosomal complexes in these cell bodies, which can be seen as Nissl granules under the light microscope.
Sucrose gradient ultra-centrifugation was effective in separating ribosomes not involved in protein synthesis (i.e., present in monoribosomal fraction) from ribosomes that were engaged in synthesis (i.e., in polyribosomal fractions).
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