One important stage in spermatogenesis is the meiotic division that occurs when the
primary spermatocyte develops into spermatid.
However in diabetic+200 rats the number of different germ cells was increased compared to diabetic group and also the number of
primary spermatocyte and spermatid cells were significantly increased compared to diabetic group.
Nucleus of Sertoli cells and germ cells (spermatogonia type A, pachytene
primary spermatocyte and round spermatid) were counted in 20 seminiferous tubules per rat (n = 10 animals [group.sup.-1]) at stage VII of spermatogenesis, under a light microscope (Leica DMLS), at x 400 magnification.
In a study, administration of 200 and 300 mg/kg to rats for 60 days showed a significant increase in the number of sperms, sertoli cells, and
primary spermatocyte compared to the control group.
Spermatogenesis was also divided into five stages: spermatogonia,
primary spermatocyte, secondary spermatocyte, spermatids and spermatozoa.
Under normal physiological conditions, the
primary spermatocyte (in preleptotene) migrates through the junctions of the Sertoli cells from the basal membrane to the tubules intraluminal compartment.
This latter cell gives rise to the
primary spermatocyte, which quickly undergoes its first meiotic division and passes through 4 prophase stages, leptotene, zygotene, pachytene, and diplotene, before undergoing meiotic metaphase.
Moreover, in our study, proacrosomal vesicle formation occurs in the spermatid stage, whereas Sousa and Oliveira (1994) report its occurrence in the
primary spermatocyte. In the paper by Sousa and Oliveira (1994), cells described as
primary spermatocytes appear to be spermatids, based on the appearance of chromatin [ILLUSTRATION FOR FIGURES 3 AND 4 OMITTED] and the position and presence of centrioles associated with mitochondria [ILLUSTRATION FOR FIGURE 4 OMITTED].
There statistically significant (P<0.01) changes in the multinuclear structure in spermatogenic cells, predominantly in
primary spermatocyte cells in the walls of some tubules and necrotic spermatogenic cells with eosinophilic cytoplasm and pyknotic nucleus were observed (Figure 3c).
It is possible that sulfasalazine by creating free radical (Ros) and mutant in testis tissue, especially the sensitive cells of spermatogonia,
primary spermatocyte, spermatid and spermatozoa, cause serious damage and loss of these cells.
The variable appearance of a
primary spermatocyte nucleus corresponds to the various configurations of meiotic prophase.