template RNA

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Noun1.template RNA - the template for protein synthesistemplate RNA - the template for protein synthesis; the form of RNA that carries information from DNA in the nucleus to the ribosome sites of protein synthesis in the cell
ribonucleic acid, RNA - (biochemistry) a long linear polymer of nucleotides found in the nucleus but mainly in the cytoplasm of a cell where it is associated with microsomes; it transmits genetic information from DNA to the cytoplasm and controls certain chemical processes in the cell; "ribonucleic acid is the genetic material of some viruses"
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PROCEDURE FOR HCV-RNA ISOLATION: Template RNA and Reverse primer DNTPS and reverse transcriptase+Enzyme buffer were subjected to heating at 42[degrees]C for 1 hour to yield CDNA which was taken and treated as DNA template.
NoV and HAV were detected by real-time reverse transcription-PCR (RT-PCR) by using the Platinum Quantitative RT-PCR Thermoscript 1-step system (Invitrogen, Carlsbad, CA, USA) (25 [micro]L final volume) with 5 [micro]L of template RNA, and primers, probes, and conditions as described (7).
Thus 3 ug is a minimum amount of template RNA that gave excellent results for PCR amplification.
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