Trephining was done and circular piece of bone was removed from cup of trephiner. The meninges were incised by a nick with scalpel blade and enlarged with scissor.
The skin tissue samples collected by corneal trephiner at 7th, 14th, and 08th day post-treatment in different groups for evaluation of the extent of wound healing by studying the histopathological characteristics Biopsy specimens were preserved in 10% buffered formalin.
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