Streptococcus pneumoniae was detected by lytA real-time PCR (selective for autolysin
gene), and serotyping was performed by a nanofluidic real-time PCR that detects 50 serotypes as individual serotype/serogroup including all vaccine serotypes (6).
Enterococcus faecium biofilm formation: identification of major autolysin
AtlAEfm, associated Acm surface localization, and AtlAEfm-independent extracellular DNA Release.
Nowadays efforts are being made to investigate virulence genes coding for pneumolysin (ply) and autolysin
(lytA), as these two antigens have been linked up with virulence of S.
A major autolysin
of Pseudomonas aeruginosa: subcellular distribution, potential role in cell growth and division, and secretion in surface membrane vesicles.
Characterization of the importance of Staphylococcus epidermidis autolysin
and polysaccharide intercellular adhesin in the pathogenesis of intravascular catheter-associated infection in a rat model.
This assay utilized the autolysin
gene and was evaluated using only a small number of culture-negative clinical samples.
Their exact mechanism of action is unknown but likely involves effects on the bacterial cell envelope and/or induction of autolysin
activity and inhibition of protein synthesis.
Auto, a surface associated autolysin
of Listeria monocytogenes required for entry into eukaryotic cells and virulence.
Polymerase chain reaction: Several PCR assays using different target genes of pneumococcus, including pneumolysin, autolysin
, DNA polymerase 1, PBP 2B, and PsaA, have been evaluated.
Capsular polysaccharide/adhesin and cell-surface-localized autolysin
(atlE) are two of the key proteins that enable the surface attachment of S.