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Related to autoradiogram: autoradiography


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Students are reminded that nucleic acid is not normally visible on the filter at this point, but upon adding a probe that is, for example, specific for the molecule represented by the red dye, that molecule will be detected on an autoradiogram.
Historically, the standard approach to measuring mtDNA content is Southern blot analysis with radioactive probes for both mtDNA and nuclear DNA, followed by densitometric analysis of the autoradiogram or phosphoimage (18).
sup32p]dCTP-labeled Cp3-13 probe, and viewed by autoradiogram.
Make also a drawing of the autoradiogram for control mice and transgenic mice that contain two copies of the transgene.
2) Band intensity of the autoradiogram shown in Figure 1, are arbitrary units given by image analyzer.
Figure 2B shows the original IS6110 RFLP autoradiogram image of the lane with the additional band and two representative images from other laboratories.
To keep the focus sharply on this issue, it considers only cases in wh is no doubt as to the existence and measurements of the bands on the autoradiogram.
Each isoform on the blot was visualized with a horseradish peroxidase-conjugated goat anti-mouse antibody, and the signal was detected on an autoradiogram by chemiluminescence as described above.
Positive samples containing HSV show a characteristic "retarded" band on the autoradiogram of the electrophoresis gel.
d) Measurement of the mean TRF length by quantitative densitometry of the smear pattern of an autoradiogram may not be accurate.
An autoradiogram of allele-specific gene expression studies by restriction isotyping in our five patients is given in Fig.
This method, currently based on the use of membrane as solid support [24,25] and autoradiogram analysis, is tedious and seems difficult to be done by laboratory technicians unskilled in molecular biology.