In the same paper, it was opined that it is often difficult to distinguish histologically between granulomatous reactivation due to lepra reaction and leprosy relapse, and that bacterioscopy
alone is useful in such situations.
Each participant also underwent a clinical and gynecological examination for genital specimen collection for bacterioscopy
, cytology, and the detection of CT DNA by hybrid capture (QIAGEN Group Corporation).
Physical parameters evaluated were volume, color, aspect and density and, for the sedimentological analyses, microbiological aspects were evaluated by GRAM bacterioscopy
, and a Neubauer chamber was used for the total leukocytes and blood cell count.
On 11/01/2004, it was carried out bacterioscopy
native strokes separated vesicles--microbial cells in the visual field have not been diagnosed.
The main laboratory analysis included the bacterioscopy and smear sputum culturing with drug sensitivity testing (DST) for anti-TB drugs of the first and the second lines, the complete blood account, general urine account, biochemical analysis, blood' urea, serum creatinine level, liver function tests (transaminases, serum bilirubin levels) which were conducted monthly.
In particular, data of sputum bacterioscopy for MBT, roentgenological data at hospitalization and in dynamics were to be given.
, histopathology of freshly prepared cytology smears and virological investigations were used routinely in diagnosing the infectious agents.
7) Tests included bacterioscopy
by Gram staining; motility (SIM), production of cytochrome oxidase test; TSI reaction; arginine hydrolysis, and lysine and ornithine decarboxylation; halophilic differentiation test (at 0, 3, 6, 8 and 10% NaCl); acetoin production (Voges-Proskauer test); growth at 42[degrees]C; hydrolysis of onitrophenyl-[beta]-D-galactopyranoside (ONPG); carbohydrate fermentation (glucose, mannitol, lactose, sucrose, arabinose, and mannose), and the indole production test.
A direct bacterioscopy with Gram staining was conducted which revealed Gramnegative bacillus forming palisades or simple chains in a typical disposition in the polymorphonuclear cytoplasm, on material obtained from chancre pus.
Ineffective treatment was determined if the lesions were still ulcerated at that time and with they presented positive bacterioscopy, in which case rescue treatment was prescribed.