Biuret

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Bi´u`ret


n.1.(Chem.) A white, crystalline, nitrogenous substance, C2O2N3H5, formed by heating urea. It is intermediate between urea and cyanuric acid.
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In the assay of serum proteins by the biuret reaction, dextran can cause turbidity due to formation of an insoluble complex of dextran with copper in strongly alkaline solution, leading to spurious hyperproteinemia.
Total protein concentration was assayed by microplate biuret reaction, the readings being made with a Modulus Multimode Microplate Reader (Turner Biosystems).
Biuret reaction has been extensively studied and reagent has been found active in the determination of acidamides, acid-imides and amidines [17, 18].