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carnitine |
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No references found | As explained, the acetyl-CoA is attached to L-carnitine by means of the enzyme carnitine acetyltransferase I. In feeding studies in old rats, these mitochondrial metabolites and antioxidants improve the age-associated decline of ambulatory activity and memory, partially restore mitochondrial structure and function, inhibit the age-associated increase of oxidative damage to lipids, proteins, and nucleic acids, elevate the levels of antioxidants, and restore the activity and substrate binding affinity of a key mitochondrial enzyme, carnitine acetyltransferase. Materials and methods Chemicals and reagents Berberine, ethylenediaminetetraacetic acid (EDTA), 2-aminofluorene (AF), N-acetyl-2-aminofluorene (AAF), dithiothreitol (DTT), Tris, acetylcarnitine, leupeptin, bovine serum albumin (BSA), phenylmethylsulfonylfluoride (PMSF), dimethyl sulfoxide (DMSO), acetyl-coen-zyme A (Acetyl-CoA) and carnitine acetyltransferase were obtained from Sigma Chemical Co. |
carnitine acetyltransferase |
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