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cefoperazone |
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In their studies, the Michigan researchers first treat mice for several days with the broad-spectrum antibiotic cefoperazone to destroy the gut flora. In the selective agar method, the fecal suspension (approximately 1 g/mL of saline) was plated onto Butzler's medium comprising Mueller-Hinton agar (Oxoid Ltd, Basingstoke, United Kingdom) containing 5% sheep blood and the antimicrobial supplement cefoperazone 30 mg/L, rifampicin 10 mg/L, and amphotericin B 2 mg/L (Institute Virion, Ruschlikon, Switzerland) (13). 064% sodium cefoperazone in brucella broth) (11) were added to the suspension, gently mixed, and incubated under microaerobic atmosphere at 37[degrees]C for 4 h, then at 42[degrees]C for 48 h. |
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