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cell-free |
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Viability relative to control was calculated by the absorbance at 450 nm corrected for the cell-free blank media absorbance. An additional flask was also prepared in which the original inoculum was incubated under the same experimental conditions but within a cell-free environment. Laboratory work undertaken to identify the mechanism showed that a cell-free extract prepared from the microorganisms decreased the length of pulp fibers that are the principal components of primary sludge. |
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