Using qPCR, we showed the measurement of nonself DNA to have high specificity, low imprecision, and high reliability for linear quantification of DNA chimerism
down to 16 GE.
Quantification of chimerism
is used to monitor engraftment of donor cells following stem cell transplantation and is essential for early diagnosis of graft failure or relapse of the disease.
Myeloid cell chimerism
analysis performed 6 months post transplantation showed 99% donor chimerism
, according to 6 alleles (ABI 310 genetic analyzer).
The presence of a discriminatory allorecognition system implies that there are costs and benefits to chimerism
that are correlated to relatedness, but the nature of these costs and benefits is still poorly understood (reviewed in Grosberg, 1988; Rinkevich and Weissman, 1992; De Tomaso, 2006)
is rare in humans: [approximately equal to] 40 cases have been reported.
In addition to complete revision of the existing material, several new chapters have been added to the second edition on bioinformatics; microarrays; methylation assays; FISH; laser capture microdissection; quality assurance; chimerism
studies for bone marrow transplantation; and the ethical, legal, and social issues of genetic counseling.
1 Rating chimerism
performed in patients undergoing allogeneic transplantation using the method fragment length analysis using a set of AmpFISTR NGM sequencer.
This patient received a nonmyeloablative transplant and had bone marrow chimerism
Presence of donor- and recipient-derived DNA in cell-free urine samples of renal transplantation recipients: urinary DNA chimerism
However, graft rejection is the undesired possible outcome following mixed chimerism
Random amplified polymorphic DNA (RAPD) analysis reveals extensive natural chimerism
in a marine protochordate.
In addition to the routine genotyping at codons 136154 and 171, PRNP gene sequences from the 2 scrapie cases were determined by sequencing of PCR amplificates from brain-derived DNA to compensate for the possibility of a genetic chimerism