Total cholesterol was determined using the cholesterol esterase
method on a semi-automated analyser.
3, detergent 1, cholesterol esterase
, cholesterol oxidase, peroxidase, 4-Amino-Antipyrine and ascorbic acid), non LDL lipoproteins are solubilized by detergent one and release cholesterol is subject to enzymatic reaction to be eliminated.
For cholesterol, the assay consisted of an enzymatic, calorimetric method whereby cholesterol esters were cleaved by the action of cholesterol esterase
to yield free fatty acids and cholesterol.
TC was assessed with cholesterol esterase
and cholesterol oxidase by using enzymatic colorimetric method.
These methods use combinations of surfactants, proteins, and ionic components to selectively discriminate between HDL-C lipoproteins and other lipoproteins in their reaction with cholesterol esterase
1982, "Hydrophobic ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase and cholesterol esterase
," Journal of Biochemistery.
For estimation, extraction of each sample was done with one disc only and 50 [micro]l of the extract was added to 1 ml of the commercially available enzymatic reagent kit containing cholesterol esterase
, cholesterol oxidase, peroxidase, phenol and 4-amino antipyrine in PIPES buffer (Lot no.
Enzyme induced biodegradation of polycarbonate-polyurethanes :dose dependence effect of cholesterol esterase
We determined the amount of free and esterified cholesterol (total cholesterol) using a colorimetric method (Roche) in the presence of cholesterol oxidase and cholesterol esterase
and then measured the absorbance at 405 nm.
One of those enzymes, cholesterol esterase
, softens the polymers, or resins, used in dental work, Shiu Heman Tsang of the University of Toronto in Ontario reported last week at the American Association for Dental Research in San Antonio.
hydrolysis esterified cholesterol to free cholesterol.
Reagent 1 consisted of 600 U/L cholesterol esterase
, 500 U/L cholesterol oxidase, 1200 KU/L catalase, 2 mmol/L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, sodium salt, and dihydrate in PIPES buffer (50 mmol/L, pH 7.