Procured is a device for the detection and quantification of proteins in histological preparations with the ability to remove multispectral autofluorescencnE[degrees]ho background and modulation of individual fluorescently labeled markers and chromogen
, supplemented with software enabling image analysis in terms of localization of various tissue types and their characteristic areas by means of instrumental learning including colocalization and quantification of the detected markers in relation to selected areas.
The RedMap kit (Ventana Medical Systems) served as the substrate chromogen
Aldehydes react with p-anisidin form a chromogen
which absorbs at a wavelength of 350nm, which can be measured by spectrophotometry .
Amino sugars when heated in an alkaline solution form a chromogen
, which produces a purple coloured compound when reacted with N, N-dimethyl-p-aminobenzaldehyde in an acid solution (Ehrlich reagent) whose colour is measured using a spectrophotometer at 530nm which was according to the procedure of Elson and Morgan .
The conjugated anti-rabbit IgG (Sigma-Aldrich[R]) was diluted 1:10,000 in PBSLT and after further washing, orthophenylenediamine chromogen
(OPD) (Sigma-Aldrich[R]) was added.
The medium also possesses an enhanced dual chromogen
system that ensures colonies of Salmonella are always green while non-target organisms appear black or colourless, for clear differentiation.
For the most part, the vast majority of microscopic pathology is still done by viewing or imaging one marker at a time in serial sections, using a hematoxylin counterstain and either eosin (H&E) or, for protein IF1C measurements, 3,3'-diaminobenzidine (DAB) as a chromogen
DAB was used as the chromogen
, and Mayer hematoxylin was used as the counterstain.
An aminoethylcarbizole (AEC) peroxidase chromogen
kit (Daco Corporation, Carpinteria, CA) was dripped on the slides and viewed at 10- to 20-minute intervals and put into tap water as soon as staining was observed.
Diaminobenzidinetetrahydrochloride (Fluka Chemie GmbH, Buchs, Switzerland) was used as chromogen
, and slides were counterstained with Papanicolaou solution (Merck GmbH, Darmstadt, Germany).
In addition, Harlequin Listeria Chromogenic Agar uses a novel chromogen
to distinguish L.
In the Morgan-Elson reaction the NAG in the reducing end is successfully transformed into a chromogen
under alkaline condition at 100[degrees] C and subsequently by the action of p-dimethyl amino benzaldehyde (p-DMAB) in glacial acetic acid and hydrochloric acid mixture (9:1; v/v) to give a reddish purple colored product that can be detected at 585 nm.