To detect calcium concentrations in culture medium, we used a calcium concentration assay kit (o-cresolphthalein complexone
kit; Teco Diagnostics, Anaheim, CA, USA) following the manufacturer's instructions.
Sankara Narayanan, TSN, Subbaiy in, M, "Sodium Diethyl-dithiocarbamate: An Effective Complexone
for Cold Zinc Phosphating.
Total serum protein and albumin were estimated by modified Biuret and Dumas method, glucose by glucose oxidase method, Alanine transaminase (ALT), Aspartate transaminase (AST) and Alkaline phosphatase (ALP) by IFCC (International Federation of Clinical Chemistry) method, Blood urea nitrogen (BUN) by glutamate dehydrogenase method, uric acid by enzymatic photometric test by IFCC method, creatinine by Jaffe's kinetic method, calcium by O-Cresophthalein complexone
method, phosphorus by modified Metol method, sodium and potassium by colorimetric method, total cholesterol (TC) by cholesterol dehydrogenase/peroxidase method, triglycerides (TG) by colorimetric enzymatic method.
Serum and urine calcium were estimated by the colorimetric method using o-cresolphthalein complexone
(Sigma-Aldrich, USA) in an alkaline medium (19).
Application of two fluorescent chemicals, alizarin complexone
and alizarin red S to mark otoliths of the Atlantic Cod Gadus morhua L.
The analytical methods employed were as follows: AST, ALT, LDH, ALP, GGT, CK, and amylase by a Japanese Society of Clinical Chemistry (JSCC) recommended method, using the Japanese certified enzyme reference material (7); TP, biuret method; albumin, bromcresol green method; TCho, TG, CRE, UN, and UA, enzymatic methods; HDL, direct enzymatic method with specific inhibition of cholesterol in other fractions; Na, K, and Cl, ion-selective electrode method; Ca, o-cresolphthalein complexone
method; and IP, molybdate method.
Otolith-marking experiments of juvenile walleye pollock Theragra chalcogramma using oxytetracycline, alizarin complexone
, and alizarin red S.
Determination of N in the needles was carried out according to the Kjeldahl method; determination of P was carried out in Kjeldahl Digest by Fiaster 5000, stannous chloride method, ISO/FDIS15681; determination of Ca in Kjeldahl Digest by Fiaster 5000, o-cresolphtalein complexone
method ISO3696; Mg was determined by Fiastar 5000, titan yellow method, ADSTN90/92; K was determined by the flame photometric method .
We prepared these materials according to the protocol provided by NIST and assayed them immediately on the Hitachi Modular, using the o-cresolphthalein complexone
method with the original assigned value used for the calcium value of the C.
Fishes were then placed in a separate tank containing 80 liters of seawater with 100 mg/L of alizarin complexone
for two hours (Thomas et al.
The most widely used method involves colorimetric detection of calcium complexes by o-cresolphthalein complexone
(o-CPC) (2,3) or arsenazo.
Because of the photosensitivity of alizarin complexone
, juveniles were immersed in 20 L of an aerated 200 mg/L solution of alizarin complexone
for 24 h in the dark.