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Congo red |
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16-18) Identification of amyloid is based on three criteria: (1) Congo red binding and green birefringence under polarized light, (19) (2) a fibril structure on electro microscopy, (20) and (3) confirmation of the characteristic cross beta-pleated sheet on x-ray crystallography and infrared spectroscopy. Sections stained with Congo red dye demonstrated the characteristic yellow and apple-green birefringence, and the lesion had the beta-pleated sheet structure and antiparallel conformation by low-angle x-ray diffraction, typical of amyloidosis. |
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