Acetic acid, Acetone, Acid fuschin, Ammonium alum(aluminium ammonium sulphate), Ammonium hydroxide, Ammonia solution, Aniline blue, Basic fuchsin, Bismark brown, Tips for micropipette 100-1000 micro litre, Calcium chloride, Charcoal, Chloroform, Citric acid, Congo red
, Copper sulphate, Chloral hydrate, Crystalline phenol, D.
5,20) In a small subset of these patients the casts stain with Congo red
, are apple green birefringent, and contain fibrillary material with characteristics most consistent with amyloid fibrils.
16-18) Identification of amyloid is based on three criteria: (1) Congo red
binding and green birefringence under polarized light, (19) (2) a fibril structure on electro microscopy, (20) and (3) confirmation of the characteristic cross beta-pleated sheet on x-ray crystallography and infrared spectroscopy.
Apple-green birefringence under polarization after staining with Congo red
is diagnostic (figure 2).
7) On a given section, 60% to 80% of vacuolated muscle fibers contain amyloid material; a fluorescence-enhanced Congo red
technique is usually required to visualize the amyloid when present in small amounts.
The vascular media was thickened; however, there was no evidence of amyloid deposition on Congo red
11] In our patient, no evidence of amyloid deposits was noted, either morphologically or on Congo red
Sections stained with Congo red
dye demonstrated the characteristic yellow and apple-green birefringence, and the lesion had the beta-pleated sheet structure and antiparallel conformation by low-angle x-ray diffraction, typical of amyloidosis.
Four-micrometer-thick, formalin-fixed, paraffin-embedded tissue sections were stained with hematoxylin-eosin, periodic acid-Schiff, Giemsa, Congo red
, and crystal violet for light microscopic examination.
was introduced in Germany in 1885 as the first economically lucrative direct textile dye.
Stains for keratin, HMB-45, leukocyte common antigen, L26, CD3, periodic acid-Schiff with diastase, smooth muscle and muscle-specific actin, S100, Congo red
, and [Lambda] light chain were all negative.
More than 100 years after its discovery, the Congo red
histologic stain is still of fundamental importance in the laboratory.