However, there are no reports on cotyledonary
leaf explants being used for regeneration studies.
Genomic DNA was isolated from unexpanded cotyledonary
leaves of three bean seedlings from each parent and each RIL line according to the protocol of Afanador and Haley (1993) with a modified CTAB extraction procedure (extraction buffer: 1.
Plantlets of Vigna radiata were regenerated from cotyledonary
Shoot regeneration was also noted on cotyledonary
leaves on some explants cultured on TDZ plus IBA containing 1.
It has been reported previously that water deficit stress can result in reduced mobilisation of cotyledonary
starch  and transport of sucrose from cotyledons to the embryonic axis .
Callus induction and plant regeneration from cotyledonary
explants of ash gourd (Benincasa hispida L.
SINCE THE FIRST TRANSGENIC SOYBEAN PLANTS were developed via Agrobacterium-mediated transformation of cotyledonary
nodes (Hinchee et al.
Combining the data from these two sources, it is possible to develop a generalized scenario for collar development in temperate trees: in angiosperms, and a few gymnosperms, the collar originates from stem tissue immediately above the cotyledonary
Micropropagation of Lupinus texensis from cotyledonary
After one month, those among them reaching the cotyledonary
stage (5-10 mm, figure 1) were transferred to MS medium supplemented with 0.
Individual plants were cut 25 mm below the cotyledonary
node, removed from the field and fractionated into stems (including branches, petioles, squares, and flowers), leaves, and bolls (fruit).
Because many monocot cotyledons have a clasping base with two thick sides flanking the growing point, some thin longitudinal sections of a cotyledonary
node can produce a two-lobed outline with a central growing point.