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Magnified endoscopic observation of early colorectal cancer by linked color imaging with crystal violet staining (with video).
Biofilm formation was measured in 96-well polystyrene microtiter plates (Costar, USA), following 24h of incubation at 35 [degrees]C, and staining with crystal violet as previously described by Stepanovic et al.
Importantly, this real-time biofilm disruption data correlated well with the data from traditional end point assays such as the labor intensive crystal violet staining technique.
After the procedure, all frogs were released and samples of peptone broth were incubated for 48 hours at 28[degrees]C, and the material was inoculated in petri dishes with Crystal Violet agar (Bacto[R]); subsequently, these were incubated at the same temperature.
A crystal violet assay was used to evaluate the effect of the organic acids on the formed biofilms.
21 % decolorization of crystal violet was achieved at a dye concentration of 40 mg/l and enzyme dose of 28 U/ml.
Some of those compounds such as crystal violet and malachite green are known for being toxic and carcinogenic, causing health disorders like mutations, cytotoxicity, neurotoxicity, and chromosomal aberrations (Oplatowska et al.
The thickness and integrity of the bacterial armor determines whether crystal violet leaves a cell purple or not.
To observe the morphology, RPBCs were stained with crystal violet (Sigma).
coli do not express strong a-glucosidase activity and appear colourless or purple due to the uptake of crystal violet.