Device specifications the device should have at least the following specifications or comparable specifications: - transmitted light microscopy and documentation with a digital color camera, - incident light fluorescence microscopy and documentation with a digital black and white camera, - confocal microscopy and documentation with a digital black and white camera, - minimum of contamination possibilities, - collection of single cells (at least 2 m) directly from the culture dish
The process removes the limit on the number of cells that can be grown in a culture dish
, which until now has been strictly confined by its surface area.
Dr Eva Latorre, who carried out the experiments at Exeter University, said: "When I saw some of the cells in the culture dish
rejuvenating, I couldn't believe it.
Whether the tumor cells are put in a culture dish
or grown in mice, they evolve changes that help them grow in this new environment.
The research builds on the Indiana University School of Medicine team's previous work with a technique called three-dimensional culture, which involves incubating stem cells in a floating ball-shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish
The final supernatant was cultured in DMEM containing 10% (v/v) FBS in 60-mm culture dish
for 10 to 14 days until colonies reached approximately 2 mm in diameter.
When you put them in a culture dish
, they behave very differently.
Thirty-six vegetable seeds were placed neatly on each culture dish
and the temperature was controlled at 30C using a thermostat.
When Assisted Hatching is performed using a laser it does not have to be removed from its culture dish
and placed in a separate "hatching plate".
Our experimental design uses a collimated light source directly above a culture dish
that illuminates half the dish while the other half is kept dark.
In a culture dish
, yes, there are all kinds of things we can do to make that happen.
Researchers simply place their culture dish
on to the stage and then access all controls using the LCD touchscreen, tapping the screen to capture images and pinching with their fingers to zoom up to 20x magnification.