Objective: Curli are functional amyloid fibers that constitute the major protein component of the extracellular matrix in pellicle biofilms formed by Bacteroidetes and Proteobacteria.
coli curli biosynthesis and address the fundamental questions concerning the molecular processes that allow the spatially and temporally controlled transport and deposition of these pro-amyloidogenic polypeptides.
qRT-PCR was used to investigate the transcription levels of curli genes (csgA and csgB), motility genes [flhD and motB), Shiga-like toxin genes (stxl and stx2), and quorum-sensing genes (IsrA and luxS) in E.
Most notably, the gene expression of the csg operon (csgA and csgB), which is involved in curli formation, was markedly repressed by coumarin, umbelliferone, and esculetin (Fig.
This puts control of curli
fiber production in the hands of the researchers, who can adjust the amount of AHL in the cells' environment.
Previously determined SNP patterns T/G/T/A or G/T/C/C at Sakai genome positions 337,933 (ECs0320, putative receptor), 1,460,599 (ECs1414, curli
production assembly/transport component), 2,370,797 (ECs2397, transport system permease protein), and 5,404,166 (ECs5279, fimH-locus) have been shown to be cluster specific (12).
The amyloids go into fibers known as curli
that are extruded by the bacteria to strengthen the structures of biofilms.
Elam is also studying the possible disease-causing effects of bacteria that produce curli
Curli knows well the literature on the same phenomenon in other European countries and places the Italian case in this larger historiographical picture.
One must notice at the outset that when the author speaks of work she refers almost exclusively to factory or white collar jobs: female work in agriculture or in domestic industry--still, as Curli recognizes, t he most important sectors of female employment during the war--is briefly referred to at the beginning of the volume, but it is not the object of any detailed investigation.
Expression of curli was not affected by cranberry extract, the assessment of P fimbriae expression was not reliable due to extract-induced agglutination of erythrocytes.
Effect of subMlCs of cranberry extract on curli expression of planktonic cells was assessed according to Hammar et al.