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cyprinid |
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From hatching, fry were fed newly hatched Artemia until approximately 60 dph, when commercial (estrogen-free) cyprinid food pellets were introduced (Calverton Fish Farm, Nottingham, UK). Measurement of vitellogenin, a biomarker for exposure to estrogenic chemicals, in a wide variety of cyprinid fish. For our studies we employed the fathead minnow as a model cyprinid species. |
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