In vitro amplifications of a fragment of the cytochrome oxidase
subunit I (COI) mitochondrial gene were carried out using the universal primers previously described by Folmer et al.
To confirm the validity of the species by molecular methods, we sequenced a fragment of the cytochrome oxidase
subunit I (COI) gene of mitochondrial DNA of Liza affinis and Liza klunzingeri from coastal waters of China and Pakistan, respectively.
One of the vital enzymes in respiratory chain is cytochrome oxidase
(COX) that produces water without ROS production by reaction with oxygen (24).
Nine new species-locus-specific primers for three different loci of mitochondrial DNA, namely D-loop, cytochrome oxidase
subunit I (COI), and cytochrome b, were successfully designed.
Myocardial cytochrome oxidase
activity is decreased following carbon monoxide exposure.
A fragment of the cytochrome oxidase
I gene was amplified by PCR from the extracted nucleic acids using primers LCO1490/HC02198 and following the protocols by Folmer et ai (1994).
knowlesi populations in Malaysia differed and independently became zoonoses, we focused on 2 genes that have been extensively used for phylogenetic studies (13-16): 1 nuclear, encoding the type A small subunit ribosomal 18S RNA (PkA-type 18S rRNA), and 1 mitochondrial, encoding the cytochrome oxidase
subunit I protein (PkCOX1).
The serum and liver cytochrome P450 (P450), cytochrome oxidase
2A6 (CYP2A6) and cytochrome oxidase
2E1 (CYP2E1) were measured using the enzyme-linked immunosorbent assay kits from Wuhan Beinglay Biotechnology Corp.
This way a novel photorespiration, where PSI can substitute for cytochrome oxidase
, is created.
In addition to being a component of oxygen-carrying hemoglobin, iron is a cofactor for cytochrome oxidase
, which is essential for mitochondrial energy production through its role in the electron-transport chain.
Two DNA barcode regions, the ITS nuclear rDNA region and the cytochrome oxidase
subunit I (COi)mitochondrial gene, were sequenced for identification of Leohumicola species.
GADPH, Lamin A/C and cytochrome oxidase
subunit IV (Cox IV) were used as loading reference for cytoplasmic protein, nuclear protein and mitochondrial protein, respectively.