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cytopathic |
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Although the specimen exhibited enterovirus-like cytopathic effect by inoculation into HEF and GMK cells when cell culture system for virus isolation was used (2), extracted RNA from the supernatant of the cells showed no amplification by reverse transcription--PCR (RT-PCR) when 1 of the conventional primer sets for human enteroviruses was used (3,4). We suspected a malignancy, but histopathology of biopsy specimens revealed that the mass exhibited the classic signs of the HSV cytopathic effect, including the presence of intranuclear inclusion bodies, ballooning degeneration of epithelial cells, and a ground-glass appearance of some nuclei. is a three-way bovine viral diarrhea (BVD) vaccine that includes noncytopathic (NCP) Type 1, NCP Type 2 and Cytopathic Type 1. |
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