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dGTP |
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A 10-[micro]L mix containing 4 [micro]L reverse transcription buffer (Invitrogen, Cergy Pontoise, France), 1 [micro],L dNTP (dATP, dTTP, dCTP, and dGTP, 25 mM each; Pharmacia, Saclay, France), 2 [micro]L 0. Two microliters of the DNA preparation was amplified in a 50-[micro]L reaction mixture containing 200 [micro]mol/L of each primer, 200 [micro]mol/L (each) dATP, dCTP, dGTP, and dTTP (Invitrogen), 1. 5 mmol/L magnesium chloride (Bioline); 200 [micro]mol/L each of dATP, dTTP, dGTP, and dCTP;, 300 nmol/L of each primer pair, 0. |
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