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The first G peak of codon 12 is twice as high as the subsequent T peak because the G peak represents the light produced from the extension of 2 dGTPs (2x), whereas the T peak represents the incorporation of only one dTTP (1X) into the elongating strand.
dATP, dCTP, dTTP, dGTP--1,25 mM each--were used to amplify intron 1 with Taq DNA polymerase.
Labeling was obtained using a PCR procedure with the following dNTP concentrations: 200 [micro]M dATP, 200 [micor]M dCTP, 200 [micro]M dGTP, 150 [micro]M dTTP, and 50 [micro]M dig-11-dUTP (Roche Molecular Biochemicals).
2], 0, 2 [micro]M de cada oligonucleotideo (G1 e G2), 100 [micro]M de dNTP (dATP, dTTP, dCTP e dGTP) e 2,5U, de Taq DNA Polimerase (Ludiwing [R]).
Polymerase Chain Reactions (PCR) were carried out in a final volume of 25 [micro]l containing 20 ng template DNA, 100[micro]M each dATP, dTTP, dGTP & dCTP, 20 ng of decanucleotide primers, 1.
For PCR amplification of the CYP3A43 rs501275 polymorphism we used the primers F 5'-GTCAATGGCAATTTTCTGTT-3' and R 5'-CTGTCTTCACAAACCAGATG-3' in a 50 [micro]l reaction mix containing 50 ng of genomic DNA, 120 ng of each of the forward (F) and reverse (R) primers, 200 [micro]M of dATP, dCTP, dGTP and dTTP, 1.
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification; VRE = Vancomycin Resistant Enterococcus.
Downie's second article, appearing in the Winter 2004 issue of DttP, begins with a summary of the many perceived barriers to incorporating government documents into literacy instruction, but it offers solutions as well.
Omission of dATP, dTTP, or dGTP during the telomerase extension step of the TRAP reaction resulted in no elongation products being amplified; however, omission of dCTP did not affect the formation of a ladder pattern of telomerase products (data not shown).
1 mM each of dATP, dGTP, dTTP and dCTP, one unit of Taq DNA polymerase (Perkin Elmer, Norwalk, Conn.
2], 200 [micron]M de cada dGTP, dATP, dTTP, dCTP (Promega, Madison, WI), O,5 [micron]M de cada iniciador (5'-ATCCAAGACAACACTA CTAA-3' y 5'-AAATATCCTCA AAGTTCC-3') y 2,5 unidades de Taq polimerasa (Corpogen, Colombia).
2], 200 [micro]M each dATP, dCTP, dGTP and dTTP, 1 [micro]M each primer and 1 unit of DNA polymerase (BioTools Labs, Madrid, Spain) in a final volume of 25 [micro]l.
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