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denature |
Also found in: Medical, Legal, Encyclopedia, Wikipedia, Hutchinson | 0.01 sec. |
denature [diːˈneɪtʃə], denaturize, denaturise [diːˈneɪtʃəˌraɪz] vb (tr) 1. to change the nature of 2. (Chemistry) to change (a protein) by chemical or physical means, such as the action of acid or heat, to cause loss of solubility, biological activity, etc. 3. (Chemistry) to render (something, such as ethanol) unfit for consumption by adding nauseous substances 4. (Physics / Nuclear Physics) to render (fissile material) unfit for use in nuclear weapons by addition of an isotope denaturant n denaturation n
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| Each PCR cycle consisted of annealing at 68[degrees]C for 1 min, primer extension at 72[degrees]C for 5 min, and denaturation at 94[degrees]C for 1 min. The micronization process prevents the beans from losing their flavor as a result of heat denaturation. 30 sec of template denaturation at 92[degrees]C if using 3'MGB quencher, or at 95[degrees]C if using 3'TAMRA quencher (step 3), and 1 min of assay-specific annealing at 60[degrees]C (step 4). |
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