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denature |
Also found in: Medical, Legal, Encyclopedia, Wikipedia, Hutchinson | 0.03 sec. |
denature [diːˈneɪtʃə], denaturize, denaturise [diːˈneɪtʃəˌraɪz] vb (tr) 1. to change the nature of 2. (Chemistry) to change (a protein) by chemical or physical means, such as the action of acid or heat, to cause loss of solubility, biological activity, etc. 3. (Chemistry) to render (something, such as ethanol) unfit for consumption by adding nauseous substances 4. (Physics / Nuclear Physics) to render (fissile material) unfit for use in nuclear weapons by addition of an isotope denaturant n denaturation n
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| Real-time PCR conditions were a single hot-start cycle at 95[degrees]C for 30 s, followed by 55 cycles of denaturing at 94[degrees]C for 10 s, annealing at 58[degrees]C for 6 s (for Bartonella) or at 60[degrees]C for 6 sec (for the 4 Bartonella species), and final extension at 72[degrees]C for 10 s. Denaturing polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA). According to the government's announcement the test is a "more accurate genetic testing method, denaturing high performance liquid chromatography, or DHPLC screening. |
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