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Desiccator |
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The method that takes the least amount of time and equipment and still gives a crisp chip is using a very hot (475 degrees or higher) oven or a desiccator (a drying oven). Transfers of 10-14% inoculum (v/v) to fresh liquid NMS medium were prepared weekly along with streaking on solid plates of carbon-free Bacto agar (Difco, Detroit, MI) and NMS that were stored in an airtight desiccator filled with methane:air (30% v/v) at 30[degrees]C. The samples were weighed after cooling for 5 min in a desiccator at ambient conditions. |
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