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destain |
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Thin and thick blood smears were prepared, fixed with methanol, stained with 10% Giemsa stain for 30 rain, and destained with water (10). 005% Coomassie Brilliant Blue solution (in 40% methanol, 7% acetic acid) overnight at room temperature, then destained and dried. After electrophoresis, the gel was stained with ethidium bromide for 30 min, destained in distilled water for 1 h, and DNA was visualized under UV light (Gel doc 1000, Bio-Rad Laboratories, Inc. |
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