Praziquantel effective against Monogenea (Dactylogyrus vastator, Dactylogyrus extensus, Diplozoon paradoxum).
Effects of praziquantel, miclosamide, levamisole hydrochloride and metrifonate on Monogenea (Gyrodactylus aculeati, Diplozoon paradoxum).
The present study reports the results of molecular analysis of the Internal Transcribed Spacer (ITS) of ribosomal DNA of 3 Monogenean species namely Diplozoon kashmirensis Kaw, Diplozoon aegyptensis Fischthal et Kuntz, Diplozoon guptai  using Polymerase Chain Reaction (PCR), nucleotide sequencing and construction of phylogenetic from different fish hosts of the Kashmir valley.
Parasite specimens of three Diplozoon species were collected from fish hosts of Carassius carassius; Cyprinus carpio communis; Schizothorax curvifrons; Schizothorax esocinus; Schizothorax niger and Schizothorax plagiostomus from Wular lake, Anchar lake, Dal lake, Manasbal lake, River Jhelum and River Sindh of Kashmir valley preserved in 100% ethanol for genomic DNA extraction and stored at-200C for good quality of DNA.
The present specimens having rectangular opisthaptor with four pairs of clamps and two individuals in form of a cross belongs to genus Diplozoon Nordmann, 1832.
PCR amplification was carried out to amplify ITS region of Diplozoon species (Table 3).
GenBank: AF973616; Diplozoon kashmirensis, complete sequence.