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electrophoresis |
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electrophoresis [ɪˌlɛktrəʊfəˈriːsɪs] n (Chemistry) the motion of charged particles in a colloid under the influence of an applied electric field Also called cataphoresis electrophoretic [ɪˌlɛktrəʊfəˈrɛtɪk] adj
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| The Agilent 2100 Bioanalyzer system (Agilent Technologies, Waldbronn, Germany) was used to separate 1 [micro]L of PCR product electrophoretically. Equal amounts (20 lag of protein) of each sample were electrophoretically separated on a 10% SDS polyacrylamide gel and electro-transferred to a PVDF (polyvinylidene difluoride) membrane. We then electrophoretically transferred([double dagger])([double dagger]) the proteins at 300 mA for 60 minutes onto to polyvinylidene fluoride (PVDF) microporous membrane (Immobilon-P Transfer Membrane). |
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