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emission spectrum |
Also found in: Medical, Acronyms, Encyclopedia, Wikipedia, Hutchinson | 0.04 sec. |
emission spectrum n (Physics / General Physics) the continuous spectrum or pattern of bright lines or bands seen when the electromagnetic radiation emitted by a substance is passed into a spectrometer. The spectrum is characteristic of the emitting substance and the type of excitation to which it is subjected Compare absorption spectrum
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| 5) is performed on the emission spectrum from a reference solution with known concentration of fluorophore and the emission spectrum from a suspension of microspheres with immobilized FITC. It is widely accepted that describing a measured x-ray peak by a Gaussian distribution is a sufficient approximation to derive accurate x-ray intensities from an x-ray emission spectrum measured with an EDS. It has been found that emission spectrum matching between a reference solution and an analyte and normalization by the corresponding extinction coefficient are required for quantifying fluorescence signals using flow cytometers. |
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