dispar showed that the restriction endonuclease
XhoI cut exclusively in the E.
For example, Methanococcus jannaschii produces a specific restriction endonuclease
found to be useful in cleavage of small single-stranded DNA "flaps.
The linear DNA fragment of this event, obtained by digestion with the restriction endonuclease
MluI, contained two gene expression cassettes consisting of (i) the cry3Bb1 coding region regulated by the root-enhanced 4-AS1 plant promoter, the wheat chlorophyll a/b-binding protein (wtCAB) leader, rice actin intron, and the wheat heat shock protein (tahsp17 3') transcriptional terminator and (ii) the nptII (Beck et al.
Cheil Jedang Corporation (Korea) has patented a novel endonuclease
enzyme which is secreted from immune cell and recognizes bacterial DNA as foreign agent and processes it to produce about 10 bp single-stranded oligonucleotide including CpG motif which is involved in immune response.
Syngenta's patent provided evidence that VIP3A toxin produced apoptotic type of cell death, including the production of membrane-bound apoptotic bodies and activation of endonuclease
enzymes that cleave chromatin into discrete fragments.
The technology involves a process for the controlled amplification of at least one part of a starting DNA containing a plurality of restriction sites for a determined specific restriction endonuclease
, and of which at least part of its nucleic acid is unknown.
These variations now await further conformation through both restriction endonuclease
digest results and more complete MegaBACE sequencing using both a forward and reverse primer.
gene heredity mutation mitosis embryo allele helix clone sequence polymer autosome phenotype recessive metaphase locus polymerase telophase oligonucleotide geneticist interphase prophase phage bacteriophage chromomere transduction transformation nucleus Mendelian genome meiosis chromosome recombinant pedigree plasmid vector replicon nucleosome chromatography zygote centromere anaphase genotype endonuclease
backcross exonuclease polyploidy diakinesis cytogenetics crossover segregate codominance dominance translocation nucleolus
Thirteen restriction endonucleases
generated a total of 28 fragments; four of the restriction endonucleases
(BamHI, EcoRI, PstI, SalI) made no cuts, while another restriction endonuclease
(BglII) resulted in a single cut (Table 1).
Methods for isolation, purification, and restriction endonuclease
digestion of mtDNA followed Lansman et al.
Chromosomal digest by low-frequency cutting restriction endonuclease
and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.