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 (ĕn′dō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
Any of a group of enzymes that catalyze the hydrolysis of bonds between nucleotides in the interior of a DNA or RNA molecule.


(Biochemistry) an enzyme that is responsible for scission of a nucleic acid chain, the action of which is not confined to the terminal nucleotide. Compare exonuclease


(ˌɛn doʊˈnu kliˌeɪs, -ˌeɪz, -ˈnyu-)

any of a group of enzymes that degrade DNA or RNA molecules by breaking linkages within the polynucleotide chains.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
nuclease - general term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units
restriction endonuclease, restriction enzyme, restriction nuclease - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
References in periodicals archive ?
dispar showed that the restriction endonuclease XhoI cut exclusively in the E.
For example, Methanococcus jannaschii produces a specific restriction endonuclease found to be useful in cleavage of small single-stranded DNA "flaps.
The linear DNA fragment of this event, obtained by digestion with the restriction endonuclease MluI, contained two gene expression cassettes consisting of (i) the cry3Bb1 coding region regulated by the root-enhanced 4-AS1 plant promoter, the wheat chlorophyll a/b-binding protein (wtCAB) leader, rice actin intron, and the wheat heat shock protein (tahsp17 3') transcriptional terminator and (ii) the nptII (Beck et al.
Cheil Jedang Corporation (Korea) has patented a novel endonuclease enzyme which is secreted from immune cell and recognizes bacterial DNA as foreign agent and processes it to produce about 10 bp single-stranded oligonucleotide including CpG motif which is involved in immune response.
Syngenta's patent provided evidence that VIP3A toxin produced apoptotic type of cell death, including the production of membrane-bound apoptotic bodies and activation of endonuclease enzymes that cleave chromatin into discrete fragments.
The technology involves a process for the controlled amplification of at least one part of a starting DNA containing a plurality of restriction sites for a determined specific restriction endonuclease, and of which at least part of its nucleic acid is unknown.
These variations now await further conformation through both restriction endonuclease digest results and more complete MegaBACE sequencing using both a forward and reverse primer.
gene heredity mutation mitosis embryo allele helix clone sequence polymer autosome phenotype recessive metaphase locus polymerase telophase oligonucleotide geneticist interphase prophase phage bacteriophage chromomere transduction transformation nucleus Mendelian genome meiosis chromosome recombinant pedigree plasmid vector replicon nucleosome chromatography zygote centromere anaphase genotype endonuclease backcross exonuclease polyploidy diakinesis cytogenetics crossover segregate codominance dominance translocation nucleolus
Thirteen restriction endonucleases generated a total of 28 fragments; four of the restriction endonucleases (BamHI, EcoRI, PstI, SalI) made no cuts, while another restriction endonuclease (BglII) resulted in a single cut (Table 1).
Methods for isolation, purification, and restriction endonuclease digestion of mtDNA followed Lansman et al.
Chromosomal digest by low-frequency cutting restriction endonuclease and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.
Restriction endonuclease (restriction endonuclease)