Our experiment demonstrated the fibroblast capacity of proliferation and osteogenesis with the induction of fibronectins.
Although fibronectins play a vital role in adhesion, migration, chemotaxis and opsonization (19), many factors contribute to the local environment in a synergistic way.
It is known that the main cell component of the granulation tissue is the fibroblast and the main biomolecule of the fester-like secretion is fibronectin (3).
After the cells adhered overnight, fibronectin (Sigma, USA) was added to the medium at the following concentrations: 0 (control group), 10, 20, 40, 60, and 80 [micro]g/mL.
The fibroblasts in groups conditioned with fibronectin showed stronger proliferation compared with those of the control group.
Fibronectins are known to contribute to cell adhesion, migration, growth and differentiation .
ECM consists of several distinct components (Figure 1) which can be divided into three groups: i) structural proteins, such as collagen and elastin, ii) specialized adhesion proteins, such as fibronectin, fibrilin, and laminin, iii) glycosaminoglycans (GAG) and proteoglycans (PG).
Matrix metalloproteinases (MMPs) are a family of zinc enzymes responsible for the degradation of extracellular matrix components such as elastin, collagen, proteoglycans, laminin and fibronectin during tissue remodeling processes .
In particular, accumulation of collagen type III and V, and to a lesser degree type I collagen and fibronectin in the reticular layer has been associated with asthma [64,65].