The principle of flow cytometry is based on laser light dispersion, sensitization, and cellular detection with fluorochrome
For staining, either of two primary antibodies was used, specific for either microtubules or centrosomes, in combination with a secondary antibody containing the fluorochrome
As negative controls, the respective isotype control (BD) labelled with the same fluorochrome
Bacterial cells was enumerated by epifluorescent microscopy (Axiosp 1, Zeiss, triple filter Texas Red-DAPI-fluorescein isothiocyanate, 1000x magnification) and using fluorochrome
fluorescein diacetate and UV-radiation (Kepner & Pratt, 1994).
1 Cycle Sequencing Kit (Applied Biosystems, USA), comprising DNA polymerase, a mixture of deoxyribonucleotide triphosphates, and fluorochrome
labeled dideoxynucleotide triphosphate.
Islet cells were stained with acridine orange-propidium iodide (AO-PI) fluorochrome
(Sigma Chemical, USA) and assayed for survival rate.
When whole blood is added to the monoclonal antibody reagent, the fluorochrome
labelled antibodies in the reagent bind specifically to leucocyte surface antigens.
2008) described the G-banding pattern and studied also fluorochrome
staining and fluorescence in situ hybridization of telomeric and rDNA probes.
malmeanum (SCM 60) clones stained with the DAPI fluorochrome
Conjugation of fluorescein isothiocyanate fluorochrome
with purified HBP and confirmation of incorporation of SP-HBP to semen
Image quantification of fluorescence relies upon applying substances that are luminescent under ultraviolet (UV) light, like Glo Germ or fluorochrome
, to the hands (Hautemaniere, Diguio, Daval, Hunter, & Hartemann, 2009; Turner et al.
According to the method, the changes of intensity of orange fluorescence of monocyte/macrophage-labeled monOC10nal antibody (PE Mouse Anti-human CD163 or PE Rat Anti-human IL-10, BD Pharmingen, BD Bioscience, USA), conjugated with fluorochrome
phycoerythrin (PE), were measured.