The smears were stained with ZN method using 1% carbol fuchsin
, 25% sulphuric acid and 0.
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In the latter study, the large neurons were the ones staining stronger both with paraldehyde fuchsin
and against serotonin, which is in accordance with the present observation in neurons of Scrobicularia plana.
Modified aldehyde fuchsin
staining showed that pancreas of the diabetic rats had irregular islet with decreased secretory granules in beta cells, but changes of pancreatic islet in the animals were minimized by administration of extract.
All specimens were smeared on clean scratch-free new glass slides and stained by carbol fuchsin
, the classic Z-N stain, which requires heating.
was used for staining nematodes within the root systems whereas phloxine B was used to stain Meloidogyne spp.
Nematode staining in plant tissue in experiments 1 and 2 was performed in the laboratory by using the acid fuchsin
method (BYRD et al.
5+ Growth in presence of dyes Thionin 20 [micro]g/mL - - Basic fuchsin
20 [micro]g/mL - - A + + M - -
Tissue sections of 5um thickness were obtained to stain with haematoxylin and eosin stain (HandE) and aldehyde fuchsin
stain for beta cells and immunohistochemical (IHC) stain using Bandeiraeasimplicifolia (BS)-1 lectinto stain intra-islet capillaries.
Sections were stained with new Fuchsin
substrate chromogen (DCS Innovative Diagnostik-Systeme) and hematoxylin.
After this period, the samples were washed in distilled water, transversal cut freehand with a razor blade, stained with double staining, astra blue and basic fuchsin
(Roeser, 1962), and mounted on semipermanent histological slides.
After incubation, 2-3 [micro]l of carbol fuchsin
dye to stain Mycobacterium cells and crystal violet to stain epithelial cell were added to each tube and the mixture was gently shaken.