The slide-mounting process was as follows: (a) after making a small incision on the back of papaya mealybug, it was heated in 10% NaOH for 10 min; (b) the body contents was expelled and the body was gently flattened with a microspatula; (c) the preparation was stained morphologically in a saturated solution of fuchsine
in a mixture of distilled water, lactic acid and glycerol (1:1:1) for 3-5 h; (d) washed in glacial acetic acid for 1 h to stabilize the staining, transferred to lavender oil, allowed to stand for at least 1 h, and (f) placed in a drop of Canada balsam on a slide and covered with a cover glass.
2], fixed in 10% formaldehyde, embedded in paraffin, sectioned at 10 [micro]m, and stained with hematoxylin of Carazzi and eosin, and a trichrome staining (VOF, according to Gutierrez 1967) of hematoxylin of Carazzi, light green, orange G, and acid fuchsine
The strain grew on Brucella agar supplemented with 5% horse serum in the presence of basic fuchsine
, thionine, and growth on safranin O.