Pancreas was removed and fixed in 10 % buffer formalin, and histological slides were prepared and stained with H & E and modified aldehyde fuchsine
method (Noorafshan et al.
Light microscopy (LM): According to Wodehouse method (1935), pollen samples were taken from plants and slides were prepared by using basic fuchsine
FNA material obtained was smeared on slides and directly stained with strong Carbol fuchsine
and steamed for five minutes.
2]O/l) prior to fixation in 10% formaldehyde, embedded in paraffin, sectioned at 10 ([micro]m, and stained with hematoxylin of Carazzi and eosin, and a trichromie staining of hematoxylin of Carazzi: light green, orange G and acid fuchsine
It might consist of a combination of different triphenyl-methane dyes such as fuchsine
and crystal violet, solvents and mordants like ethanol, acetone and lugol's iodine as reagents of Gram staining as the most extensively performed test in microbiology.
The slide-mounting process was as follows: (a) after making a small incision on the back of papaya mealybug, it was heated in 10% NaOH for 10 min; (b) the body contents was expelled and the body was gently flattened with a microspatula; (c) the preparation was stained morphologically in a saturated solution of fuchsine
in a mixture of distilled water, lactic acid and glycerol (1:1:1) for 3-5 h; (d) washed in glacial acetic acid for 1 h to stabilize the staining, transferred to lavender oil, allowed to stand for at least 1 h, and (f) placed in a drop of Canada balsam on a slide and covered with a cover glass.
Transversal freehand sections were made at middle third of the blade, followed by staining with basic fuchsine
diluted to 0.
Than the fuchsine
used for the ZN stain, demonstrating that the first stain has advantages to the second as it is a more effective diagnosis method, of faster execution and reading, so it is mostly used in a large number of samples or for epidemiological studies.