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glyceraldehyde |
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To specifically identify virus replication, a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was used to simultaneously amplify glyceraldehyde 3 phosphate dehydrogenase (G3PDH), SARS-CoV genomic RNA (gRNA), and subgenomic RNA (sgRNA) (10). To test the validity of our approach, two oligonucleotides were identified as being the best-performing probes to evaluate the mRNA level for the rat glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Values were reported as an average of triplicate analyses normalized to glyceraldehyde phosphate dehydrogenase RNA levels. |
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