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guanidine |
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Viral RNA was extracted from 250 [micro]L of the 10% fecal suspension by using the guanidine isothiocyanate method and the Rnaid Spin Kit (BIO 101, Anachem Bioscience, Bedfordshire, UK) according to the manufacturer's instructions, with slight modifications (16). Regarding the relationship of the guanidine activator DPG to the thiuram activator TBzTD, an "inverse activator composition" (IAC) has to be established. HBECs were lysed in guanidine isothiocyanate (GITC) buffer [4 M GITC (Boehringer Mannheim, Indianapolis, IN), 25 mM sodium citrate (pH 7. |
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