The authors were able to identify eleven crude extracts inhibiting the enzymatic activity of the viral neuraminidase and four of them were also able to show inhibition of haemagglutination
Diagnosis can be made by serological testing during the convalescent phase using haemagglutination
inhibition, complement fixation, and through mass tag polymerase chain reaction.
After centrifugation (20 min, 1,200 x g), the serum was aliquoted into 1-mL vials and stored at -20[degrees]C for serum antibody measurements using haemagglutination
inhibition test (Alexander, 1988).
Immunogenicity of synthetic angiotensin II octapeptide and analysis of antibodies by passive haemagglutination
The role of Casoni's skin test and indirect haemagglutination
test in the diagnosis of hydatid disease.
The effects of the combination were compared to several positive controls (acyclovir, ribavirin and amantadine hydrochloride) and assessment of the effects was done by standard viral viability tests (plaque reduction assay, cytopathogenic assays, virus titrations, analysis of the viral proteins in virus-specific enzyme immunoassays, and haemagglutination
2mg 2/5 3/5 60 Positive - 5/5 0/5 0 Control Negative - 0/5 5/5 100 Control Toxicity 20gm 0/5 5/5 100 Control Key: D = No of dead mice N = No of mice inoculated MHA = Median haemagglutination
titre Table 3: Inhibition of yellow fever virus replication in chick embryo by aqueous extract of Gynostemma pentaphyllum Inoculation Extract Egg MHA litre % regimen concentra- mortality (Log 2 inhibition controls tion (D/N) reciprocal) (mg/mL) allantoic Pre 200 0/5 1.
Data included urinalysis, blood count, indirect haemagglutination
test (IHA), abdominal ultrasonography (AUS), intravenous urography (IVU) and computerized tomography (CT).
The rest of the routine laboratory tests including renal function tests, liver function tests, random blood sugar (RBS), Treponemal pallidum haemagglutination
assay (TPHA), serum cryptococcal antigen (CRAG) titres, hepatitis B surface antigen (HBsAg) and brucella agglutination assay were unremarkable.
Unique Physico-chemical properties of Japanese B encephalitis antibody virus haemagglutination
of India, Kolkata), Treponema pallidum haemagglutination
assay (Plasmatec TPHA test kit, Hansard Diagnostics, United Kingdom) in VDRL reactive cases and fluorescent treponemal antibody absorption (FTA-ABS) test using FTA-Abs IgG and IgM IFA (Viro-Immun Labor-Diagnostika Gmbh, Oberursel & Virgo, Calbiotec, USA) in sera giving discrepant results in the above two tests were carried out.
The indirect haemagglutination
assay (IHA) for hydatid disease was negative.