Trials revealed that using a flow rate of 3 mL/min and an injection volume of 500 [micro]L created optimal conditions for hemocyanin
1) Partial hemocyanin
gene (H1) Haliotis tuherculata HmG16D (AJ252741.
Briefly, peptides were conjugated via mmaleimidobenzoylN-hydroxysuccinimide ester to keyhole limpet hemocyanin
, and sheep were immunized with 100 ~g of peptide, initially in its conjugate form, and with 50 ~g in 4-week intervals thereafter.
BiovaxID is comprised of a tumor-derived protein (tumor-specific antigen) linked to KLH (keyhole limpet hemocyanin
, a carrier protein) and administered with GM-CSF (granulocyte macrophage colony stimulating factor).
The high demand of Cu by crustaceans may be a result of the fact that up to 40% of the Cu load in shrimp is bound in hemocyanin
(Depledge 1989), indicating the close association of Cu with the oxygen-carrying pigments in crustaceans.
In blue crabs, hemocyanin
is an oligomer composed of six different monomer subunits.
Briefly, peptides were conjugated with m-maleirnidobenzoyl-Nhydroxysuccinimide ester to keyhole limpet hemocyanin
In California, its economic importance is a result of its hemocyanin
(keyhole limpet hemocyanin
), which is an extracellular protein used in immunology, with potential oncological therapeutic applications (Harris & Markl 1999, Harris & Mark1 2000).
Molecular cloning of insect pro-phenol oxidase: a copper containing protein homologous to arthropod hemocyanin
A peptide representing amino acid residues 980-993 (DKIPEKNNDYMNKW) of the BRCA2 sequence was synthesized (Anaspec) and conjugated to keyhole limpet hemocyanin
for immunization as described previously (31).
2_110 13 Cytidine deaminase HmC4778_234 14 Fasciclin domain-containing protein HmSNP4691_183 14 Heat shock protein 70 Hm3Dl0_1 15 Hemocyaninlike HmC4791_1099 15 Hypothetical protein HmC1449_847 16 Insulinlike growth factor binding protein 7 HmC18774_676 16 3-Hydroxy-3-methylglutaryl-CoA HmC22449_261 16 Protein disulfide isomerase HmC3835_411 16 Sorbitol dehydrogenase HmC45_3002 16 Hillarin HmC6061_1289 16 Phosphoglycerate mutase 2 HmC929_2563 17 Hexokinase Hm3B4_2 18A Ribosomal protein 1 Hm3B4_7 18A Ribosomal protein l HmC253_1545 18C PDZ and LIM domain protein Zasp Hmid2044 18C Hemocyanin
Isoform 1 HmidILL2.
meets chitons: phylogeny of polyplacophorans revisited by hemocyanin