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heterologous |
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| The heterologously produced histidine (His)--tagged GlpQ fusion protein was purified by using Ni-NTA spin columns (Qiagen, Valencia, CA, USA), and the protein concentration was determined with Bradford assay (Bio-Rad Laboratories, Hercules, CA, USA). 50] values of 60 and 80 nM on Candida albicans sterol 14[alpha]-demethylase and of 580 and 610 nM on human sterol 14[alpha]-demethylases, both enzymes heterologously expressed in yeast (Lamb et al. |
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