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histidine |
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histidine [ˈhɪstɪˌdiːn -dɪn] n (Life Sciences & Allied Applications / Biochemistry) a nonessential amino acid that occurs in most proteins: a precursor of histamine
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The heterologously produced histidine (His)--tagged GlpQ fusion protein was purified by using Ni-NTA spin columns (Qiagen, Valencia, CA, USA), and the protein concentration was determined with Bradford assay (Bio-Rad Laboratories, Hercules, CA, USA). To examine the requirement for a positive charge for MAs(III) uptake, Arg-219 was changed to alanine, lysine, glutamate, and histidine. The new plant, which has an annual amino acid production capacity of 2,500 tons, manufactures 12 different amino acids including arginine, histidine and serine for transfusions. |
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